Carotenoid synthesis and pleiotropic effects in carotenoid-deficient seedlings of maize

摘要

Plastid-envelope membranes from seedlings ofZea maysL. made carotenoid-deficient by either norflurazon treatment or mutation lack an activity permitting conversion of phytoene to β-carotene. This activity in membrane fractions was measured by coincubation in vitro with a soluble system from spinach chloroplasts capable of converting 14Cisopentenyl pyrophosphate into phytoene. When grown in light, the carotenoid-deficient seedlings lack many soluble chloroplast proteins, including NADP-dependent malic enzyme (EC 1.1.1.40), pyruvate phosphate dikinase (EC 2.7.9.1), and ribulose-1,5-bisphosphate carboxylase (EC 4.1.1.39), but apparently still contain the soluble activities permitting synthesis of phytoene