Conformational aspects and functions of tRNA

摘要

In the process of protein biosynthesis, an aminoacyl-tRNA synthetase strictly recognizes the cognate amino acid and tRNA species. Spectroscopic and biochemical analyses have been made of a heterologous system ofThermus thermophilusglutamyl-tRNA synthetase andEscherichia colitRNAGlu. The conformational difference between the initial complex and active complex has been observed, which is probably related with the strict recognition of aminoacyl-tRNA synthetase.