A Comparison of Two Methods for the Determination of Lactic Acid in Muscle

摘要

SUMMARYTwo methods of determining lactic acid in mammalian tissues are compared. Lactic acid was estimated by the procedure of Barker and Summerson and by the enzymatic test with lactic dehydrogenase. The standard error of the mean at the 5 level was 5.8 for the former and 3.1 for the latter. Values for lactic acid found by both methods did not agree in most cases. Differences of over 40 were detected. After chromatographic purification of the extracted lactic acid, the Barker and Summerson values were identical with the enzymatic values. Lactic acid isolated by thin‐layer chromatography was identified by its infrared spectrum as thep‐phenyl‐phenacylester. The enzymatic test should be regarded as the more reliable procedure for determining lactic acid in