A new ELISA for the quantification of equine procalcitonin in plasma as potential inflammation biomarker in horses

摘要

In human medicine, procalcitonin (PCT) is a very common and well-established biomarker for sepsis. Even though sepsis is also a leading cause of death in foals and adult horses, up to now, no data about the role of equine PCT in septic horses has been available. Based on monoclonal antibodies targeted against human PCT, we report here the development of a sandwich ELISA for the quantification of equine PCT in equine plasma samples. The ELISAwas characterized for intra- and interassay variance and a working range from 25 to 1,000 ng mL~(-1) was defined as within this range; both intraand interassay variances were below 15 . The target recovery ranged between 73 and 106 . The ELISA was used to determine the equine PCT concentration in 24 healthy and 5 septic horses to show the potential for clinical evaluation of equine PCT. Significantly different (P=0.0006) mean equine PCT concentrations were found for the healthy control group and the sepsis group (47 and 8,450 ng mL~(-1)).