Improving Transformation Efficiency of Arabidopsis thaliana by Modifying the Floral Dip Method

摘要

Arabidopsis thaliana transformation with the floral dip method was improved by modifying the cell density and mode of application of the Agrobacterium inoculum. Drops of inoculum were applied 4 times to closed flower buds. The inoculum OD6QO was increased from 0.8 to more than 2.0. These modifications improved reproducibility and increased transformation efficiencies to 2-3.