Molecular form of pancreatic elastase 1 in human plasma

摘要

In order to study the molecular forms of immunoreactive pancreatic elastase 1 (IRE) in human plasma, we investigated the characteristics of proelastase 1, elastase 1, and their α1-protease inhibitor (α1-PI) complexes on anion-exchange (Mono Q) chromatography, gel (Superose 12) chromatography, and enzyme immunoassay systems with monoclonal antielastase 1 antibodies of different affinities for α1-PI-elastase 1 and α1-PI-proelastase 1 complexes. The rate of complex formation between proelastase 1 and α1-PI was dependent on temperature of incubation and concentration of α1-PI. At 37°C, 90 of proelastase 1 was bound to α1-PI during 2 h of incubation of purified human proelastase 1 with human α1-PI. Similar results were also obtained by 2 h of incubation of proelastase 1 with human plasma at 37°C. Therefore, under physiological conditions, neither free proelastase 1 nor elastase 1 can be detected in the human blood stream, and most IRE exists as the complex of proelastase 1 with α1-PI in hu