Genomic DNA Analysis of Rat Retinal Tumor Induced by Adenovirus Type 12

摘要

To elucidate the pathogenesis of human retinoblastoma, we investigated the genomic expression in retinal tumors induced by human adenovirus type 12 in rats, using various DNA probes. Seven rats received a single intraocular inoculation of concentrated virus fluid within 24 hours after birth. Intravitreous tumors were induced in two out of seven animals (28.5) within 30 to 64 days after the inoculation. A remarkably uniform histologic feature, i.e., neuroblastic cells in association with Homer‐Wright pseudorosettes, was present in all cases. The adenovirusrelated oncoprotein geneE1Aand human retinoblastoma susceptibility gene were detected in the tumors by Southern blot hybridization.In situhybridization analysis demonstrated expression of adenovirus type 12E1Agene in the inner granular layer of the retina. It was suggested that integration of adenovirus type 12E1Afragment with the host genome and expression of the gene were required for induction of this tumo