Healing effect of a spray containing Rhealba (R) oat colloidal extract in an in vitro reconstitution model of skin

摘要

abstract_textpBy using a reconstituted skin model, we aimed to evaluate the efficacy of a spray containing Rhealba(R) oat extract on the rapidity of healing and the extension of the newly formed epithelium. A dermal equivalent was first made in a petri dish by combining skin fibroblasts with collagen type I. Then a punch biopsy as a source of epidermal cells was implanted on this dermal equivalent, where a multilayered epidermis developed. The spray containing Rhealba(R) oat extract was added to the culture medium to evaluate epidermal growth by immunohistochemical analysis of mitotic activity (5-bromo-2'-deoxyuridine BrDu incorporation). The extension of the neoepithelium in comparison with untreated reconstituted skin over 22 days was evaluated histologically. On day 12, 16 of positive BrDu basal cells was detected after spray treatment in comparison with 4.2 positive cells in untreated reconstituted skin (p 0.05). During epidermal differentiation between days 12 and 22, we observed a significant increase in the number of cellular epithelial layers after 16 and 18 days of spray treatment. Moreover the extension of re-epithelialization was also significantly increased after spray treatment on days 16 and 18. In conclusion, our results demonstrate a positive modulation of re-epithelialization on a newly formed epithelium by a spray containing Rhealba(R) oat extract displaying a healing effect./p/abstract_text