An LED-Based Illuminator for Microphotometric Studies of Cells

摘要

A fiber-optic illuminator with superbright semiconductor LEDs for single-cell microphotometry is described. The illuminator provides excitation at wavelengths of 385, 440, 480, and 530 nm (30, 70, 50, and 20 mW, respectively). The long-term instability of its radiant power is ≤0.1%/h, and its service life is 30000 h. The characteristics of the LED-based illuminator are compared to those of a commercial illuminator based on a DeltaRAM scanning monochromator (Photon Technology International, United States).