The female gametophyte ofTaxuswas excised at the mature seed stage and was successfully grownin vitrowithout the embryo. The gametophyte tissue, which had large lipid reserves when placed into culture, showed cell dedifferentiation. In about 6 of cultures, unorganized, crumbly outgrowths were produced. After 2 months, a slow-growing, undifferentiated tissue was isolated. It contained some isolated tracheids, and, in most cells, a rough photosynthetic apparatus was apparent. Journal translation
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