Capillary electrophoresis with field-enhanced stacking for rapid and sensitive determination of strychnine and brucine

摘要

A new capillary electrophoresis procedure with field-enhanced stacking concentration for the analysis of strychnine and brucine is established. After optimization of the separation and concentration conditions, the two alkaloids can be separated within 5 min and quantified with high sensitivity (The detection limits were 1.0 ng mL~(-1) for strychnine and 1.4 nag mL~(-1) for brucine). The method was useful for qualitative and quantitative analysis of strychnine and brucine in Strychnos nux-vomica L with recovery of 105.1 for strychnine and 98.4 for brucine.