Evidence for an active hydrophobic form of factor H that is able to induce secretion of interleukin 1‐β or by human monocytes

摘要

AbstractWe studied the capacity of human factor H to promote the secretion of a lymphocyte‐activating factor (LAF) by human monocytes cultured under serum‐free conditions. Presence of LAF in the culture supernatants was assessed with the mouse thymocyte assay. Highly purified factor H alone had no effect on thymocyte proliferation. When monocytes were cultured with factor H for 24 h, a significant secretion of LAF was observed. The effect was dose dependent over a range of factor H concentrations from 1 to 15 μg/ml. Polymyxin B did not abrogate the capacity of factor H to induce LAF secretion. Adsorption of factor H preparations onto anti‐factor H‐Sepharose completely suppressed the phenomenon. Conversely, the activity was recovered in the acidic eluate. Furthermore, factor H subpopulation Φ2, that was able to bind to phenyl‐Sepharose, was a stronger inducer of LAF secretion by monocytes than the subpopulation Φ1 (which did not bind to phenyl‐Sepharose). Using a specific radioimmunoassay for interleukin 1‐β (IL 1β), we observed a good correlation between the LAF activity and the amount of IL 1β secreted by human monocytes stimulated with factor H.We have shown previously that factor H (Φ2) bound specifically on Raji cells whereas factor H (Φ1) did not. These results argue for the participation of the interaction of factor H with its receptor to stimulate the secretion of IL 1 by monocytes and that the Φ2 form of factor H is a ligand for the h