SummaryTheSaccharomyces cerevisiaeallergens were characterized by IgE‐immunoblotting with serum samples of 83 patients; 63 represented patients with atopic dermatitis with previous positive skin prick test or RAST forS. cerevisiae, seven patients with AD but negative test results and 13 were non‐atopic controls. Disrupted whole body extract ofS. cerevisiaewas used in the assays. From the patients tested 41 patients with atopic dermatitis appeared positive in IgE immunoblotting revealing 22 IgE stained bands. From these bands 10 represented intermediate allergens, and 12 minor allergens. The most frequent staining was obtained with the 48 kD band (39). When the staining pattern of 45 kD and 48 kD bands and mannan was compared withCandida albicansallergens or purified baker's yeast enolase a simultaneous binding was seen with the 48 kD band ofS. cerevisiaeand the 46 kD band ofC. albicansand enolase whereas the 45 kD band was neither associated with the 46 kD band ofC. albicansnor purified enolase. High molecular weight staining was found in five samples. The staining pattern was associated with the mannose containing structures in parallel withC. albic
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机译：摘要对83例患者的血清样本进行IgE免疫印迹分析酿酒酵母过敏原;63 例代表既往皮肤点刺试验或 RAST forS 阳性的特应性皮炎患者。酿酒师，7 例 AD 患者但检测结果为阴性，13 例为非特应性对照。破坏全身提取物。酿酒酵母用于测定。从接受检测的患者中，41 例特应性皮炎患者在 IgE 免疫印迹中呈阳性，显示 22 条 IgE 染色条带。在这些条带中，10 种代表中间过敏原，12 种代表次要过敏原。最常见的染色是 48 kD 条带 （39%）。当将 45 kD 和 48 kD 条带和甘露聚糖的染色模式与白色念珠菌或纯化的面包酵母烯醇化酶进行比较时，观察到与 48 kD 的 S 条带同时结合。酿酒师和 46 kD 波段的 C。白色念珠菌烯醇化酶，而 45 kD 条带与 C 的 46 kD 条带无关。Albicansnor纯化的烯醇化酶。在5个样品中发现高分子量染色。染色模式与甘露糖含有与C平行的结构相关。阿尔比克
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