The distribution of the different esterases was investigated in human gingiva. The α naphthyl acetate method showed increased activity of non‐specific esterase in the region of the granular layer in orthokeratotic gingiva. A similar enzyme distribution was noted with the naphthol AS‐LC method, which suggests that the two methods are demonstrating the same enzyme. The latter method was, however, not as satisfactory as the former due to crystal formationndoxyl acetate esterase activity was also increased in the region of the granular layer. This increased activity seems to indicate that the esteraset are related to cytoplasmic autol
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