The Semi-Preparative Separation of Peptides on Reversed Phase Silica Packed into Radially Compressed Flexible-Walled Columns

摘要

The semi-preparative separation of underivatised peptide mixtures from the tryptic digestion of thyroid and pituitary proteins has been accomplished on a 10dp spherical octadecyl-silica stationary phase, Radial Pak A/C18, packed into flexible-walled polyethylene cartridges (10 #xD7; 0.8cm). With volatile ionic modifiers, such as ammonium bicarbonate, excellent resolution and peptide recoveries were obtained with this support