Attenuation ofwhitegene expression in transgenicDrosophila melanogaster: Possible role of a catalytic antisense RNA

摘要

AbstractWe have transformedDrosophila melanogasterwith a DNA construct encoding a catalytic antisense RNA directed against the primarywhitegene RNA transcript. Total RNA isolated from transformed flies was shown to catalyze the specific cleavage of in vitro transcribed substrate RNA, indicating the expression of a functional ribozyme. Moreover, transgenic lines carrying homozygous copies of this construct depict a further clear‐cut reduction in eye pigmentation when present in a genetic background that has a priori reduced levels ofwhitegene expression. © 1993Wiley‐Liss,