Crystalline selenite substituted carbonated hydroxyapatite nanorods: Synthesis, characterization, evaluation of bioactivity and cytotoxicity

摘要

Synthesis of pure and selenite substituted hydroxyapatites (HAP and Se-HAP with 0.02-0.10 Se) by sol-gel method and evaluation of their morphological features, nature of functional groups, phase purity, in vitro bioactivity and cytotoxicity are addressed. Spectral studies confirm the incorporation of selenite into the HAP lattice, accompanied by an increase in CO32- content to maintain the charge imbalance following replacement of P5+ with Se4+, thus making the formation of selenite substituted carbonated HAP. Selenite substitution in the HAP lattice has led to a higher crystallinity and increased the crystallite size. The morphology of HAP is changed from sphere to rod-like structure upon substitution by selenite and the size of the rod is increased with an increase in the selenite content. Among the Se-HAPs, a better in vitro bioactivity and cell viability are observed for 0.02 Se-HAP and 0.04 Se-HAP while the trend is reversed when the extent of selenite substitution becomes higher.