Nitrogenase activity, nitrogenase synthesis, and the growth of four species ofAzospirillumwere examined at 30°C and 42°C.Azospirillum brasilenseSp 7 did not synthesize nitrogenase at 42°C nor was the enzyme stable at this temperature. InA. lipoferum708 andA. brasilense12S the nitrogenase activity was stable at 42°C but the synthesis of nitrogenase was reduced. InA. brasilenseSp 9 the nitrogenase activity was stable and showed the highest C2H2reduction activity at 4
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