In vitroantibody response of primed rabbit peripheral blood lymphocytes to Group A variant streptococcal polysaccharide

摘要

AbstractPeripheral blood lymphocytes obtained from rabbits at various times after a primary course of immunization with streptococcal Group A‐variant vaccines were successfully stimulatedin vitrowith this vaccine. Day 5 cultures contained about 81 IgM and only 19 IgG plaque‐forming cells, day 10 cultures contained only 38 IgM and 62 IgG plaques, and day 15 cultures contained predominantly IgG plaques (83 ) and far fewer IgM plaques (17 ). Concomitantly, IgM and IgG secretion into the media was shown. Analysis of the secreted IgG antibodies by isoelectric focusing showed in principle that the clones stimulatedin vitrowere those that had determined thein vivoresponse. These data demonstrate the circulation of clonal progenitor cells in the rabbit peripheral bl