A simple. quick and sensitive procedure is described to monitor urinary clearance of aromatic amines in industrial workers. Estimation is based on the measurement of coloured Schiff's bases developed with 4-dimethylaminocinnamaldehyde-organic acid reagent. Extraction of amines in water saturated butanol ensured 89#x2013;97 recovery from biological samples with a coefficient of variation of less than 8. Regression analyses of the absorbance-concentration curves with different aromatic amines showed excellent correlation coefficient and a high molar absorptivity with the lowest detection limits ranging between 0.05 0.12#x3BC;g ml#x2212;1. The proposed reagent has additional use as a spray reagent to detect amine spots on chromatograms. Low final acidity ensures estimates of free amines without causing hydrolysis of conjugated amines.
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