High-resolution banding in chromosomes of B lymphoblastoid cells and cultured skin fibroblasts

摘要

High-resolution banded chromosomes were obtained in Epstein-Barr virus-transformed lymphoblastoid cells and cultured skin fibroblasts by aphidicolin synchronization, followed by inhibition of chromosome condensation by ethidium bromide. With this method, the chromosomes of 33.3 of lymphoblastoid cells and 48.7 of skin fibroblasts were high-resolution banded, and their mitotic indices were 1.5 and 2.7 times higher than those of control cultures.