Quantitative HPLC Analysis of Plasma Amino Acids as Orthophthaldialdehyde/Ethanethiol Derivatives

摘要

A reverse phase high performance liquid chromatographic method for the analysis of plasma amino acids is described. The method employs pre-column derivatization with o-phthaldialdehyde using ethanethiol as the reducing agent. The analysis shows good linearity and reproducibility. An average overall difference of 12 was seen for results obtained by the HPLC method versus those obtained with an amino acid analyzer. The chromatographic parameters of buffer concentration and column temperature were also examined.