Anti‐tumor necrosis factor receptor and tumor necrosis factor agonist activity by an anti‐idiotypic antibody

摘要

AbstractTumor necrosis factor (TNF) is a cytokine which, among other properties, is a principle mediator of inflammation and septic shock. It acts upon target cells by binding to specific cell surface receptors. A10G10 is a murine monoclonal antibody which recognizes human TNF and neutralizes its activity. A rabbit polyclonal antibody directed at the antigen‐binding site of A10G10 was raised and affinity purified over an A10G10 column. The resultant anti‐idiotypic antibody recognized not only A10G10 but also both TNF receptors. It showed TNF agonist activity in two different TNF bioassays, and competed with several anti‐TNF receptor monoclonal antibodies and TNF itself for binding to cells. These results represent an example of a method for obtaining antibodies to a ligand‐specific receptor in the absence of the receptor