Absorption spectra and their fourth derivatives of chloroplasts isolated from 16 different rice chlorina mutants were determined at liquid nitrogen temperature. The results suggest that Chlbis absent from 10 mutants labelled chlorina-1 to -10, while 6 mutants named chlorina-11 to -16 contain low levels of Chlb. Low temperature fluorescence emission spectra indicate that a light-harvesting Chla/bprotein of photosystem I is present in chlorina-11 to -16 but not in chlorina-1 to -10. Reinvestigation of Chla/bratios by a sensitive fluorescence method shows that the 16 mutants are divided into three groups different in the degree of Chlb-deficiency; chlorina-1 to -10 totally lack Chlb(Type I), chlorina-11 to -13 have Chla/bratio of about 10 (Type II-A) and chlorina-14 to -16 have the ratio of about 15 (Type II-B).
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