Basic strategies of molecular biological research in plant nutrition ‐ a review

摘要

AbstractA review is given on basic methodical strategies of molecular biological research in plant nutrition. Membrane transport protein identification and analysis by the use of cDNA libraries and functional expression in yeast mutants andXenopusoocytes is demonstrated. The various methods of gene isolation and analysis, including molecular data of model plants, are pointed out. The significance of research on transgenic plants permitting overexpression of introduced genes, antisense technique and analysis of promoter/reporter gene constructs is emphasized. The numerous examples of recent research simultanously indicate contemporary and future topics for molecular biological research in plant nutrition.