Adsorption of Human Reagins to Ethylchloroformate Polymerized Antigen E and Desorption with Thiocyanate Ions

摘要

Ethylchloroformate polymerized ragweed antigen E was employed as immunoadsorbent for separation of reagins from human ragweed atopic serum. For elution of adsorbed reagins, 2 m KSCN was found to be a more effective reagent than 2 m KI. Recoveries of 25–60 of the initial serum reagin content were obtained under optimal conditions of adsorption and desorptio