Plant regeneration from mesophyll protoplasts ofDiplotaxis muralis, a wild crucifer

摘要

Mesophyll protoplasts from leaves of aseptically grown shoot tips ofDiplotaxis muraliswere isolated (6.2–7.1×105protoplasts/g fresh weight of tissue) using one step enzyme digestion. The protoplasts (71 viability) underwent divisions (4.2+0.1) on plating in M8PS2medium and ultimately formed calli with 0.45+0.03 plating efficiency. Plant regeneration could be achieved both through embryogenesis and organogenesis. The efficiency of plant regeneration through organogenesis was 9 times higher than embryogenesis. Forty eight out of 52 plants regenerated so far from 3 independent experiments were normal with respect to fertility and meiotic chromosomal behavi