A simple haemolytic method for quantitation of the delta endotoxin of Bacillus thuringiensis subsp. israelensis from crude samples

摘要

A simple haemolytic assay method for quantitative estimation of the delta endotoxin ofBacillus thuringiensissubsp.israelensisfrom a crude preparation has been developed. The method has several advantages over mosquito‐larvicidal methods of assay as it is inexpensive, highly sensitive and easier to run and can be used for performing a reasonably large number of assays rapidly with high precision and with a coefficient of variation that does not exceed 1.96