Optimization of ginseng cell culture in airlift bioreactors and developing the large-scale production system

摘要

Ginseng cell suspension culture was established in 51 capacity balloon type airlift bioreactors using Murashige and Skoog medium supplemented with 7 mgl(-1) indole butyric acid, 0.5 mgl(-1) kinetin and 30 gl(-1) sucrose. Various parameters such as types of bioreactors (cylinder, balloon, bulb and cone type bioreactors), aeration volume (0.05, 0.1, 0.2, 0.3 vvm constant air supply or the amount air supply was increased from 0.05 to 0.3 vvm at 6-day intervals), and inoculum density (40, 60,80 and 100 gl(-1)) were optimized. Balloon type airlift bioreactors, aeration volume of 0.1 vvm, and inoculum density of 60 gl(-1) were found suitable for accumulation of ginseng cell biomass and ginsenosides. Based on optimized results, 5001 capacity large-scale drum type and balloon type bioreactors were established for the production of ginsenosides. Optimal yield of 187 kg and 400 kg fresh biomass and 6.2 kg and 13.3 kg dry biomass, 7.86 mg g(-1) DW and 7.75 mg g(-1) DW total ginsenosides could be achieved in 5001 capacity drum and balloon type bioreactors respectively. These results are useful for commercial production of ginseng cell biomass and secondary metabolites