Rapid and simple determination of selenium in blood serum by inductively coupled plasma-mass spectrometry (ICP-MS)

摘要

An inductively coupled plasma mass spectrometer (ICP-MS) with a rapid sample-preparative procedure was used for the determination of selenium in blood serum. Blood serum was prepared by dilution in an acidic solution consisting of nitric acid (1), X-triton (0.1) and 1-butanol (0.8). A calibration curve was established for 1-40 μg mL~(-1) (r~2 > 0.99). The limit of detection was 0.5 μg mL~(-1). Repeatability and intermediate precision were satisfactory with relative standard deviations (RSD) of 2.0 and 3.2, respectively. This method was easily applied to reference materials with satisfactory accuracy. Good correlation (r~2 = 0.96) was observed between ICP-MS and atomic absorption spectrometry (AAS) for the determination of ~(82)Se in blood serum from 23 patients. These results suggest that the sample preparative procedure coupled with ICP-MS can be used for the routine determination of ~(82)Se in human blood serum.