An improved method for the determination of glycosyltransferases usingpara-nitrophenyl-glycosides as substrates

摘要

Due to their easy purification nitrophenyl-glycosides have become of increasing interest as acceptor substrates for the assay of glycosyltransferase activity. In this communication reversed-phase chromatography is shown to provide a convenient means for the isolation of the radioactively labelled nitrophenylglycoside(s) formed in this reaction, superior to the methods hitherto used with regard to speed and specifity.