Determination of soyasaponins in Emphasis Type='Italic'Fagioli di Sarconi/Emphasis beans ( Emphasis Type='Italic'Phaseolus vulgaris/Emphasis L.) by LC-ESI-FTICR-MS and evaluation of their hypoglycemic activity

摘要

AbstractSoyasaponins are oleanene-type triterpenoid saponins, naturally occurring in many edible plants that have attracted a great deal of attention for their role in preventing chronic diseases. The aim of this study was to establish the distribution and the content of soyasaponins in 21 ecotypes ofFagioli di Sarconibeans (Phaseolus vulgaris, Leguminosae). High-performance reversed-phase liquid chromatography (RPLC) with positive electrospray ionization (ESI(+)) and Fourier transform ion cyclotron resonance (FTICR) mass spectrometry (MS) in conjunction with infrared multiphoton dissociation (IRMPD) was applied for the unambiguous identification of soyasaponins Ba (m/z959.5213, C48H79O19+), Bb (m/z943.5273, C48H79O18+), Bd (m/z957.5122, C48H77O19+), and Be (m/z941.5166, C48H77O18+), which are the only commercially available reference standards. In addition, the several diagnostic product ions generated by IRMPD in the ICR-MS cell allowed us the putative identification of soyasaponins Bb' (m/z797.4680, C42H69O14+), αg (m/z1085.5544, C54H85O22