Alloantisera can detect some of the variants of HLA DRw13 identified by oligotyping*

摘要

Abstract:Oligotyping has revealed a considerable polymorphism, pf HLA DRwl3; so far, 5 alleles coded by DRBI have been identified. An even greater number of haplotypes are apparent when considering the association of DRw13 with alleles coded by DRB3 and DQBI. Serology can now define some of these variants which had formerly escaped detection. We have tested by serology and by oligoprobes 66 genotyped Caucasoid cells comprising the known DR and DQ alleles. Among the 28 DRw6 cells, 10 different haplotype patterns were selected, of which 8 concern DRwl3 and 2 DRwl4. Four variants of DRw13 were represented: DRB1 *1301, * 1302. *1303 and 1305 associated with usual and with uncommon alleles belonging to DRB3 and to DQBI. We have centered our analysis on 19 sera of the XIth Workshop defining DRw6. Two sera (639. 826) were noteworthy since they reacted with certain subtypes of DRwl3 and with DRBI *1102, *1103 only, the other DR specificities remaining negative.