Modification of the Pad‐plate Method of Determining Chlortetracycline in Egg White

摘要

SUMMARYThe sensitivity of the pad‐plate method for detecting chlortetracycline in egg white is increased by using pH 4.5 citrate buffer rather than pH 4.5 phosphate buffer as a diluent. It is also increased by allowing about four hours for diffusion of antibiotic prior to incubation of the assay plates. Under these conditions of assay, protein binding of the antibiotic was not an important facto