Morphological features were studied of callus tissues from carrot root, endive embryo, lettuce leaf petiole and stem, parsley and spinach leaf petiole, and navy bean and red kidney bean stem grown on solid C- or D-medium. The callus tissue of carrot, endive, lettuce leaf petiole and stem, parsley, and red kidney bean were irregularly lobed while the callus tissues of navy bean and spinach were hemispherical and somewhat flattened, respectively. The cultures of carrot and endive were firm and massive, while the calli of other species were friable. Callus cells of these seven species, examined with the phase microscope, varied in size and shape. The callus cells of carrot, lettuce leaf petiole and stem, navy bean, and red kidney bean were spherical, oval, and elongated. Endive callus tissue consisted of oval and elongated cells and that of parsley consisted of spherical and oval cells, while spinach callus usually consisted of spherical cells. Elongated cells ranged from 204 to 475 mu; in length by 45 to 92 mu; in width, spherical cells 62.5 to 129.8 mu; in diameter, and oval cells 118 to 172.8 mu; in length by 63 to 94.5 mu; in width. Variations were noted in the morphology and number of cell inclusions including the nucleoli, plastids, mitochondria, and starch granules. In chlorophyllous cells, the chloroplasts were generally scattered in the cytoplasm, but in spinach it often surrounded and obscured the nucleus. Varying numbers of starch granules were seen around the nucleus and in the cytoplasm.
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