A technology for the rapid acquisition of cell number and viability

摘要

A Bioanalytical instrument system has been developed that provides much of the functionality of today s sophisticated flow cytometers, but in a small, simple-to-use, and cost-effective package. With particular emphasis on mammalian cell counting andviability determination, the system has been compared to several alternative approaches. These include microscopy (hemacytometer), impedancebased cell counters, and conventional flow cytometry. Results have shown very good agreement between the system and conventional flow cytometry. Other uses for the system include analysis of surface-expressed molecules on individual cells and quantitation of apoptosis in cell cultures.