Two-Dimensional Gel Electrophoresis Software for Automated Protein Expression Level Change Analysis

摘要

Analysis of 2-D gel electtophoresis (2DGE) images has always been a considerable bottleneck in the proteomics work flow Much woik has needed to be done manually since automatic spot detection, matching, and quantification produced rather poor-qualityresults The software discussed here delivers automated processing from 1) image analysis of silver, Coumassie, and multifluoiescence stained gels to 2) the detection of up-regulated or down-tegulated spots to 3) export of the spots of interest to a variety of spot-pickets for further MS-based identification Proteomweaver software (Definiens AG, Munich, Germany) produces reliable and reproducible results, enabling large-scale proteomic studies.