Biochanin A Inhibits Lipopolysaccharide-Induced Inflammatory Cytokines and Mediators Production in BV2 Microglia

摘要

Biochanin A, one of the major isoflavonoids in red clover or cabbage, has been reported to have anti-inflammatory effects. However, the molecular mechanism underlying the anti-inflammatory effects of biochanin A has not been completely elucidated. The aim of this study was to investigate the anti-inflammatory effect and mechanism on lipopolysaccharide (LPS)-stimulated BV2 microglia. The results showed that biochanin A suppressed LPS-induced inflammatory mediators nitric oxide, prostaglandin E-2 and inflammatory cytokines TNF-alpha and IL-1 beta production. LPS-induced NF-kappa B activation was also inhibited by biochanin A. In addition, biochanin A up-regulated the expression of PPAR-gamma and the anti-inflammatory effects of biochanin A can be abolished by PPAR-gamma antagonist GW9662. These results suggest that biochanin A exerts an anti-inflammatory property by activating PPAR-gamma, thereby attenuating NF-kappa B activation and the release of pro-inflammatory mediators.