Evaluation of an atmospheric pressure ionization ion source for high-throughput analysis of drugs directly from cell culture media at high ionic strength

摘要

THE STRATEGY USED to increase throughput in the laboratory at Pfizer Central Research (Sandwich, Kent, U.K.) involved the development of an LC-MS-MS screening technique to monitor the transport of multiple compounds across Caco-2 cell monolayers. The evaluation of multiple compounds in parallel, enabled by the selectivity of MS-MS, was found to reduce the interexperiment variability associated with earlier single-compound methods. However, in a number of multiple-compound screens it was noted that some compounds could apparently enhance the absorption of others, giving eroneous results. Positive results indicated in rapid multiple-compound screens therefore require subsequent verification in single-compoond experiments.