Automated, solid-phase DNA isolation

摘要

Traditionally, DNA extraction from whole blood has been a time-consuming process. For diagnostic and forensic purposes, polymerase chain reaction (PCR) (Hoffmann-LaRoche, Nutley, NJ) has become the method of choice for the analysis of genomic DNA. Since PCR is a fast and often automated technique, genomic DNA isolation is a rate-limiting step. This paper describes an automated protocol for Dynabeads~R DNA DIRECT~(TM) (Dynal Inc., Lake Success, NY), in which a unique combination of surface chemistry and lysis solution allows binding of soluble genomic DNA to the surface of paramagnetic particles. The present paper is concerned only with blood and Buccal scrapes as sample material. The manual version of DNA DIRECT, however, is very versatile with respect to sample types.