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Reducing Cell Culture Costs By Reducing Serum Levels

机译:通过降低血清水平降低细胞培养成本

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摘要

Cell culture is a very important research tool today, but one that is also very expensive to use. A major component of that expense is fetal bovine serum (FBS), an often essential part of cell culture. Many researchers routinely supplement their culture media with 10—20% FBS for growing their cells; however, these levels may be higher than the cells require. By reducing FBS levels by 50% or more, researchers may be able to reduce costs considerably (Table 1). It should be noted that although many cell lines will do well at lower FBS levels, not all will.
机译:细胞培养是当今非常重要的研究工具,但使用起来也非常昂贵。费用的主要组成部分是胎牛血清(FBS),这通常是细胞培养中必不可少的部分。许多研究人员通常会在培养基中添加10%至20%的FBS以使其细胞生长。但是,这些水平可能高于单元所需的水平。通过将FBS含量降低50%或更多,研究人员可能能够大幅降低成本(表1)。应当注意,尽管许多细胞系在较低的FBS水平下表现良好,但并非所有人都可以。

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