Peptide De Novo Sequencing With MALDI TOF/TOF: A Simple Approach Using Sulfonation Chemistry

摘要

One of the main focuses of the scientific community, now that major advances have been achieved in the comprehension of genome organization, has become the study of proteins, including their structure and activities. Among the different techniques available, mass spectrometry has played a fundamental role and is experiencing rapid growth. Matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF)/TOF instruments (Ultraflex Bruker Daltonics, Bremen, Germany), taking advantage of the reflectron mode, combine a soft ionization process with good sensitivity and resolution.