Culture supernatants of 17 strains of the ‘Bacteroides fragilis’ group were treated with four volumes of acetone. The precipitates, after dialysis and lyophilization, were used as antigens in the double diffusion test with antisera against serotype strains of ‘B. fragilis’. In the culture supernatant of one strain we did not demonstrate the presence of serologically active substances. Sixteen preparations reacted in immunodiffusion with antiserum against ‘B. ovatus’ serotype B. Ten preparations reacted with antiserum B only and six preparations gave, additionally, precipitation lines with other serotype antis
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