Anti-neoplastic effect of Chicken Anemia Virus VP3 gene in 'in-vitro' cultured malignant tumour cells.

摘要

This study comprised of the anti-neoplastic effect of Chicken Anemia Virus (CAV) Viral protein 3 (VP3 gene/apoptin) "in-vitro" in cultured malignant tumour cells of bovines. The VP3 gene of chicken anemia virus was cloned in Plasmid DNA 3.1 (PcDNA 3.1) eukaryotic expression vector and in vitro expression of recombinant PcDNA.CAV.VP3 was confirmed. Twenty bovine tumour cases of horn and eye cancer were subjected to this study. All the animals were subjected to surgical intervention for the removal of the tumour growth under the ideal anaesthetic combination. Biopsy samples were aseptically collected for cell culture and histopathological studies. The histopathological studies of the lesions revealed squamous cell carcinoma and fibroma. The cell culture of malignant tumour was performed using standard protocol. An in-vitro study confirmed that recombinant VP3 gene induced apoptosis in cultured malignant bovine tumour cells. The result of the present study revealed that recombinant VP3 gene (also known as Apoptin) had anti-neoplastic effect and further improvements in the dose, delivery methods and delivery frequency of VP3 gene expressing recombinant vector may help to develop recombinant VP3 gene/apoptin as an anti-neoplastic drug.