Cyanidium caldariumM-8 type grown in the dark was illuminated for 3 days, and changes of its cell and cell organelle structures and of photosynthetic activity were observed quantitatively. Dark grown (DG) cells showed no photosynthetic activity and no phycocyanin. During 3 day illumination they fully recovered their photosynthetic activities as measured by Hill reaction, and also synthesized chlorophyll a and phycocyanins. Sizes of cell, cell nucleus, chloroplast and its nucleoid observed by fluorescence microscopy after staining with DAPI increased simultaneously upon illumination. The chloroplast and its ring shaped nucleoid sizes increased especially rapidly, concomitant with the recovery of Hill activity. In fully recovered cells after 3 days, a good correlation was found among the sizes of cells, chloroplasts and chloroplast nucleoids.
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