Storing pollen of two olive cultivars: 'Rasie' and 'Nabali Baladi'.

摘要

This study was conducted on 'Rasie' and 'Nabali Baladi' olive cultivars to establish short-, mid-, and long-term pollen storage technique. Pollen was stored in normal room condition, refrigerator (4 degrees C), freezer (-5 degrees C), and cryostorage (-80 degrees C) for up to 52 weeks. Pollen was in vitro germinated on solid-agar media, and its viability was tested using 2, 3, 5-triphenlytetrazolium chloride. Cryopreserved pollen under -80 degrees C gave the best results, and refrigerated and frozen pollen gave convenient results. Under room conditions, pollen of the two cultivars has lost its germinability and viability gradually, and after 52 weeks the pollen was nonviable.