Isolation and identification of Canine Parvo Virus (CPV-2) from the faecal samples of dogs in Bhubaneswar, India

摘要

The present study was undertaken to detect the presence of canine parvo virus (CPV) in the susceptible population around the city of Bhubaneswar, India. Haemagglutination Inhibition (HI) test was employed for primary screening of the faecal samples which was followed by Fluorescent Antibody Test (FAT) and Polymerase chain reaction (PCR) for confirmatory diagnosis. A total of 24 faecal and serum samples were screened from the dogs suffering from acute vomition and diarrhoea with or without blood. MDBK(Madin Darby Bovine Kidney) cell line was utilized to isolate the virus and PCR of VP1A^P2 gene confirmed the presence of virus in cell culture supernatant. Seroprevalence study of 24 serum samples employing HI test revealed 14 (58.33%) samples to be positive while FAT sensitivity was found to be on the lower side (12.5%).