Comparison of fluorescein- and 32P-labelled probes for safe detection of potato spindle tuber viroid in potato.

Priyanka Kaundal; Tiwari J. K.; Nimisha Kaushal; Deep Kumar; Gopal J.; Pandey S. K.; Garg I. D.

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Comparison of fluorescein- and 32P-labelled probes for safe detection of potato spindle tuber viroid in potato.

机译：荧光素和 32 P标记的探针在安全检测马铃薯中马铃薯纺锤块茎类病毒中的比较。

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Two methods namely fluorescein- and 32P-labelled probes were tested for detection of Potato Spindle tuber viroid in potato. Out of 94 potato microplants tested, 82 were found positive, while 12 were virus-free. On comparison with NASH, it could be seen that fluorescein-labelled probe was quite cost effective.

机译：测试了荧光素和 32 P标记的两种方法检测马铃薯中马铃薯纺锤块茎类病毒的方法。在测试的94种马铃薯微型植物中，有82种呈阳性，而12种没有病毒。与NASH相比，可以看到荧光素标记的探针具有很高的成本效益。

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来源
《Indian Journal of Horticulture》 |2012年第1期|共4页
作者
Priyanka Kaundal; Tiwari J. K.; Nimisha Kaushal; Deep Kumar; Gopal J.; Pandey S. K.; Garg I. D.;
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正文语种 eng
中图分类园艺;
关键词
crop production; plant pathogens; potatoes; Potato spindle tuber viroid; Solanum tuberosum; viroids; angiosperms; Asia; Commonwealth of Nations; Developing Countries; dicotyledons; eukaryotes; plants; Pospiviroid; Solanaceae; Solanales; Solanum; South Asia; Spermatophyta; viroids; viruses.;

机译：作物生产;植物病原体;马铃薯;马铃薯纺锤形块茎类病毒;马铃薯病毒。;

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1. Comparison of fluorescein- and 32P-labelled probes for safe detection of potato spindle tuber viroid in potato. [J] . Priyanka Kaundal, Tiwari J. K., Nimisha Kaushal, Indian Journal of Horticulture . 2012,第1期

机译：荧光素和 32 P标记的探针在安全检测马铃薯中马铃薯纺锤块茎类病毒中的比较。
2. DETECTION OF POTATO SPINDLE TUBER VIROID (PSTVd) USING FLUORESCEIN LABELED PROBE [J] . Yogita Verma, Arti Shukla, S.M. Paul Khurana, Potato Journal . 2006,第3a4期

机译：用荧光素标记探针检测马铃薯丝管病毒（PSTVd）
3. Sensitive detection of potato spindle tuber and temperate fruit tree viroids by reverse transcription-polymerase chain reaction-probe capture hybridization. [J] . Shamloul AM, Hadidi A Journal of Virological Methods . 1999,第2期

机译：逆转录-聚合酶链反应-探针捕获杂交技术可灵敏地检测马铃薯纺锤块茎和温带果树类病毒。
4. Sensor system for detection of post-harvest spoilage of stored potato tubers [C] . P T N Spencer-Phillips, B P J de Lacy Costello, R J Ewen, International Plant Protection Congress . 2007

机译：检测储存马铃薯块茎收获后腐败的传感器系统
5. Host involvement in the replication of potato spindle tuber viroid and the evolutionary relationship between plant viroids and the hepatitis delta virus. [D] . Bojic, Teodora. 2009

机译：宿主参与马铃薯纺锤块茎类病毒的复制以及植物类病毒和肝炎三角洲病毒之间的进化关系。
6. The Challenge for Rapid Detection of High-Structured Circular RNA: Assay of Potato Spindle Tuber Viroid Based on Recombinase Polymerase Amplification and Lateral Flow Tests [O] . Aleksandr V. Ivanov, Irina V. Shmyglya, Anatoly V. Zherdev, 2020

机译：基于重组酶聚合酶扩增和横向流动测试的马铃薯主轴块茎病毒域的快速检测挑战
7. Digoxigenin-labelled molecular probe for the simultaneous detection of three potato pathogens: potato spindle tuber viroid (PSTVd), potato virus Y (PVY), and potato leafroll virus (PLRV). [O] . M Wełnicki, C Zekanowski, W Zagórski 1994

机译：标记的Digoxigenin标记的分子探针，用于同时检测三种马铃薯病原体：马铃薯主轴块茎病毒域（PSTVD），马铃薯病毒Y（PVY）和马铃薯乳蛋白酶（PLRV）。
8. Comparison of algorithms for anomaly detection in safeguards and computer security systems using neural networks [R] . Howell, JA, Whiteson, R 1992

机译：使用神经网络进行保障和计算机安全系统异常检测算法的比较

Comparison of fluorescein- and 32P-labelled probes for safe detection of potato spindle tuber viroid in potato.

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