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首页> 外文期刊>Indian Journal of Horticulture >Development of in vitro propagation protocol and gus expression studies on Asiatic lily hybrids (Lilium spp.)
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Development of in vitro propagation protocol and gus expression studies on Asiatic lily hybrids (Lilium spp.)

机译:开发亚洲百合杂交种(Lilium spp。)的体外繁殖方案和gus表达研究

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摘要

In vitro propagation experiments were carried out to study the effect of media, genotype and explant on micropropagation in three cultivars (Prato, Brunello and Dreamland) of Asiatic Lilium hybrids MS basal medium supplemented with 1 mg/l NAA induced best culture establishment and bulblet multiplication in all the three cultivars of Asiatic hybrid of Lilium. Best rooting of in vitro derived bulblets was recorded in modified MS medium supplemented with 1 mg/l NAA and 90 g/1sucrose. The potting mixture of vermiculite + FYM (1:1) proved to be the best for transplantation survival of plantlets. Gus expression varied between 20.2-24.9% in these three Lilium cultivars. The surviving explant tissues failed to exhibit the gus gene expression four weeks after transfer and all of the explant tissues became brown and died by four weeks after transfer, presumably due to minimal integration of T-DNA into the Lilium genome.
机译:进行了体外繁殖实验,研究了培养基,基因型和外植体对三种亚洲百合(普拉托,布鲁内洛和梦境)品种的微繁殖的影响,补充了1 mg / l NAA的MS基础培养基MS诱导了最佳培养和鳞茎繁殖在亚洲百合的三个杂交品种中。在补充了1 mg / l NAA和90 g / 1蔗糖的改良MS培养基中记录了体外衍生的小球的最佳生根。 ver石+ FYM(1:1)的盆栽混合物被证明是最适合小植株移植存活的混合物。在这三个百合属植物中,Gus表达在20.2-24.9%之间变化。存活的外植体组织在转移后四周未能表现出gus基因表达,并且所有外植体组织都变成褐色并在转移后四周后死亡,这可能是由于T-DNA与Lilium基因组的整合程度最低。

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