In-vitro propagation in Gerbera jamesonii Bolus

摘要

Studies were conducted with 2 Gerbera varieties to standardize the protocol for a large-scale in vitro multiplication. Shoot explants of SWM and Dilmaya (1.0 - 2.0 cm long) from in-vitro grown shoots were cultured in MS medium with varying concentrations of BAP. Significant interactions between varieties and media were recorded for culture weight, shoot number, size as well as weight and leaf number and size. Maximum culture growth and callus weight were recorded at 1.0 mg/litre BAP for both the varieties. For obtaining more number of shoots, supplementation with 0.5-1.0 mg/litre BAP was found to be optimum. Significant variations were recorded for various traits between the varieties. However, Dilmaya responded better than SWM for most of the characters. The shoots were rooted in MS basal medium and planted in culture jars filled up to one-third with careers. High mortality was recorded in carriers with sand and vermiculite, while leaf-mould and soil helped in better growth and establishment. Acclimatized plants transferred to polythene bags containing equal amount of soil:sand: farmyard manure, established better with 95-100% survival. This method, involving a simultaneous acclimatization and growth is simple, giving very high survival and better growth.